Exploration of possible catabolic factors in synovial fluid through protein and micro RNA analyses.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20022
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Clinical Research Center for Allergy and Rheumatology, National Hospital Organization, Sagamihara National Hospital
• Principal Investigator: Tanaka Nobuho 独立行政法人国立病院機構(相模原病院臨床研究センター), 政策医療企画部, 研究員 (60530920)
• Research Collaborator: FUKUI Naoshi ; MORI Toshihito
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 変形性関節症 / 関節炎 / エクソソーム / 関節液 / miRNA / 滑膜

Outline of Final Research Achievements

In year 2015, we compared concentrations of 28 proteins between 16 SFs from the progressors (P-SFs) and other 16 SFs from non-progressors (NP-SFs) using Luminex. This analysis revealed that the P-SFs contain MMP-1 and MMP-3 at significantly higher concentrations compared with the NP-SFs. In year 2016, we compared miRNA profiles between the P-SFs and NP-SFs. In results, we found that the concentrations of miR-21 and miR-150 were significantly higher in the P-SFs. We next investigated relationship in concentration between the above two miRNAs and two MMPs, and found that the concentration of miR-21 or miR-150 was inversely correlated with that of MMP-1 or MMP-3. We then investigated expression of these miRNAs and MMPs in OA cartilage and synovial tissues, and found inverse correlation between the expression of either MMP and miR-21 or miR-150 in synovium. Further analyses will be conducted in future as to the origins and relationships of these miRNAs and MMPs.

Free Research Field

関節病学