Exploration into the intracerebral network of pain transmission and the possibility of prevention of chronic pain with pre-emptive analgesia using ofMRI

2018 Fiscal Year Final Research Report

• Project/Area Number: 15K20034
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: University of Toyama
• Principal Investigator: Takemura Yoshinori 富山大学, 大学院医学薬学研究部(医学), 助教 (70624922)
• Research Collaborator: ITO Hisakatsu ; HATTORI Mizuki ; KAWAKAMI Masaaki
• Project Period (FY): 2015-04-01 – 2019-03-31
• Keywords: 痛み評価 / ダイナミックプランター

Outline of Final Research Achievements

We tried to establish a more objective method in pain assessment. A bright room and a dark room were prepared as measurement environments of mice. In addition, as for stimulation conditions, items of stimulation weight, measurement time, and loading speed to reach the maximum stimulation weight of 5 g were compared at each of time points which were 8, 10, 12 and 13 weeks.
As a result, significant differences were observed when the stimulation weight and the measurement time were measured in the dark room, but no significant difference was observed in the light room. On the other hand, no difference was found between 10 and 20 seconds for the loading speed. In short, it was found that the stimulation weight and the measurement time were more stable in the bright room.

Free Research Field

痛み

Academic Significance and Societal Importance of the Research Achievements

これまでは、マウスの痛みを調べるための行動学的手法の１つとして von Frey フィラメントを用いた測定を行ってきたが、測定者による手技のばらつきが測定結果のズレに繋がるという問題があった。そこで測定者間のばらつきを極力少なくし、より客観的に評価するためにダイナミックプランターを導入し、測定条件の設定を行った。今回の結果により、今後安定した測定結果が得られると期待できる。