2017 Fiscal Year Final Research Report
Spinal cord ischemia-reperfusion injury: a microdialysis study in the spinal ventral horn
Project/Area Number |
15K20057
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Anesthesiology
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Research Institution | Osaka City University |
Principal Investigator |
SUEHIRO Koichi 大阪市立大学, 大学院医学研究科, 講師 (10735806)
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 脊髄虚血 / プレコンディショニング |
Outline of Final Research Achievements |
The present study aimed to investigate the protective effect of remote ischemic preconditioning (RIPC) against spinal cord ischemia (SCI) and reveal its mechanism by measuring the glutamate concentration using an in vivo microdialysis in the ventral horn of the spinal cord. RIPC by limb-ischemia attenuated the increase of glutamate concentration in n the ventral horn and the decrease of motor evoked potential (MEP) amplitude due to SCI, and improved the post-SCI motor function and histopathologic changes in the spinal cord. Additionally, even with RIPC conditions, pre-administration of NMDA receptor antagonist (MK-801) led to increase the glutamate concentration and decrease MEP amplitude, and also worsened neurologic and histopathologic outcomes. Our study revealed that RIPC could show a protective effect against SCI both in early and late phases, and that the NMDA receptor played an important role for this preventive effect.
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Free Research Field |
麻酔
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