2016 Fiscal Year Final Research Report
Study of the influence that the anesthetic which assumed metabolism mode analysis a key gives to the insulin secretion of pancreas beta cell
Project/Area Number |
15K20063
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Anesthesiology
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Research Institution | Kyoto University (2016) Kansai Medical University (2015) |
Principal Investigator |
Suzuki Kengo 京都大学, 医学研究科, 助教 (90734658)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | HIF-1 / インスリン / セボフルラン / イソフルラン |
Outline of Final Research Achievements |
Several animal and human studies have indicated that volatile anesthetics impair glucose-stimulated insulin secretion (GSIS). Isoflurane and sevoflurane inhibited GSIS significantly in a concentration-dependent manner. Oxygen consumption rate in MIN6 cells but to a lesser extent than the mitochondrial uncoupler, CCCP (5 μM). Isoflurane and sevoflurane significantly suppressed glucose-induced HIF-1alpha protein expression in MIN6 cells. HIF-1alpha protein expression, which was not affected by isoflurane. Inhibition of HIF-1 activity by YC-1 treatment and RNA interference-mediated knockdown of HIF-1alpha expression significantly suppressed GSIS in MIN6 cells. Both the anesthetics inhibited the glucose-induced increase of ATP, which is dependent on intracellular hypoxia-induced HIF-1 activity, and suppressed GSIS at a clinically relevant dose in MIN6 cells.
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Free Research Field |
麻酔
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