2017 Fiscal Year Final Research Report
Investigation of diagnostic marker and drug sensitivity in human renal cell carcinoma cells targeting microRNA
| Project/Area Number |
15K20091
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Tottori University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | microRNA / 腎細胞癌 / スニチニブ抵抗性 |
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| Outline of Final Research Achievements |
Microarray analysis and reverse transcription-quantitative polymerase chain reaction revealed that miR-194-5p expression was significantly decreased in sunitinib resistant-ACHN (SR-ACHN) cells when compared with that observed in ACHN cells. Transfection of miR-194-5p, though not with negative control miR, in SR-ACHN cells could significantly inhibit cell proliferation following sunitinib treatment. Western blotting demonstrated that the expression of lysosome-associated membrane protein-2 (LAMP-2), which attenuates the anti-proliferative effect of sunitinib, was significantly higher in SR-ACHN than in ACHN cells. In addition, LAMP-2 expression was suppressed by miR-194-5p transfection in SR-ACHN cells. These data suggested that miR-194-5p downregulation may be associated with sunitinib resistance via the induction of LAMP-2 expression in human RCC.
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| Free Research Field |
泌尿器腫瘍
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