Breakthrough of the management of preterm labor -Usage of highly sensitive PCR system for detecting microorganisim in Amniotic fluid-

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20133
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: University of Toyama
• Principal Investigator: Yoneda Noriko 富山大学, 大学院医学薬学研究部(医学), 助教 (80377283)
• Co-Investigator(Renkei-kenkyūsha): NIIMI Hideki 富山大学, 大学院医学薬学研究部(医学), 准教授 (50401865)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 早産 / 子宮内感染 / 迅速高感度病原微生物検出法

Outline of Final Research Achievements

Intra-amniotic(IA) infection is one of the important risk factors for preterm birth.
1.We have established newly rapid and bacteria-free polymerase chain reaction (PCR) method. It was useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in preterm labor (PTL).
2.We examined the efficacy of the use of antibiotics in PTL with intact membranes, after evaluating intra-amniotic microbes by our PCR system. In the amniotic fluid (AF) microbe-negative PTL patients, antibiotic　therapy significantly shortened the gestation period,whereas in the microbe-positive patients, appropriate antibiotic therapy (proper antibiotic selection against identified AF microbes) was significantly associated with an increase in gestation period . Appropriate antibiotic therapy in PTL with intact membranes prolonged the gestation period.

Free Research Field

周産期