Reduction of Chondroitin sulfate chains with aging is involved in formation of multi-nucleated blastomeres in mouse

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20170
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: University of Tsukuba
• Principal Investigator: SATO BAN 筑波大学, 生命環境系, 特任助教 (90443126)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: マウス着床前胚 / 発生生物学 / 卵割 / コンドロイチン硫酸 / 細胞質分裂

Outline of Final Research Achievements

Multi nucleated blastomeres (MNB) have been widely reported in in vitro cultured embryos derived from in vitro fertilization or intracytoplasmic sperm injection. The rate of chromosomal abnormalities increases as maternal age, resulting in a dramatically increased incidence of birth defects. Although the presence of MNB is considered abnormal and is possibly related various problem, the underlying mechanism of occurance of MNB is poorly understood.
To investigate the mechanism of MNB formation, we focused on two independent research topics. (1) The frequency of multinucleated blastomeres increases in chondroitin sulfate (CS) knockout embryos. (2) Several Age-related diseases cause by which the expression level of CS diminished with age.　We hypothesized that formation of MNB is caused by reduction of CS chains with aging In this study, we revealed that CS chains moiety of CSPG decide the localization of abscission molecules in mouse preimplantation embryos by using live-cell imaging.

Free Research Field

生殖生物学