Crosstalk between keratinocytes and fibroblasts : a mechanism underlying cholesteatoma induced bone destruction.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20204
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: IWAMOTO YORIKO 大阪大学, 医学系研究科, 特任研究員 (70636480)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞間クロストーク / 角化扁平上皮細胞 / 線維芽細胞 / プロスタグランジンE2

Outline of Final Research Achievements

I succeeded in generating in vivo and in vitro models for cholesteatoma tissue using keratinocytes and fibroblasts derived from mouse ear pinnae. With these models, I demonstrated that keratinocytes stimulate osteoclast differentiation through the induction of RANKL in fibroblasts. PGE2 significantly upregulated RANKL expression in fibroblasts , suggesting PGE2 is a candidate for a RANKL inducer.
Also in human cholesteatoma tissue, osteoclasts accumulated on the bone surface, and fibroblasts highly expressed RANKL.RNA sequence analysis of fibroblasts in perimatrix layer revealed highly expression of genes which involve in differentiation of osteoclasts.

Free Research Field

分子生物学