Expression and localization of MHC class I-related chain molecules A and B in human rhabdomyosarcoma cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20300
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Osama Woman's and Children's Hospital (2017); Osaka University (2015-2016)
• Principal Investigator: Yamamichi Taku 地方独立行政法人大阪府立病院機構大阪母子医療センター(研究所), その他部局等, 小児外科・診療主任 (30715165)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 横紋筋肉腫 / NK細胞 / 免疫療法

Outline of Final Research Achievements

Natural killer (NK) cells are important for the defense against tumors. The interaction of the MHC class I-related chain molecules A and B (MICA/MICB) with the corresponding NK group 2, member D receptor activates NK cells. We investigated the expression and localization of MICA/MICB in human rhabdomyosarcoma (RMS) tumors and cell lines.
The immunohistochemical staining showed that 21 of 25 clinical tumor samples were positive for either MICA or MICB. The Western blot analysis revealed that the MICA protein was presented in all RMS cell lines (RH30, RD and RMS-YM), as well as the mRNA expression, and the MICB protein was presented in the RD and RMS-YM cells. However, the surface expression of MICA and MICB was limited with only MICA being detected on RD cells.　Our results suggest that RMS cells have the ability to produce the MICA and MICB proteins, whereas only RD cells expressed MICA on their cell surface. Therefore, MICA/MICB could be therapeutic targets for RMS immunotherapy.

Free Research Field

小児外科