Elucidation of the molecular mechanisms of diabetic-derived neutrophils and exploration of lead compound that contribute to inflammation regulation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20314
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Nagasaki University
• Principal Investigator: UMEHARA Takahiro 長崎大学, 医歯薬学総合研究科(医学系), 助教 (60617421)
• Research Collaborator: IKEMATSU Kazuya ; MORI Ryoichi ; KIMBERLY Mace
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 糖尿病性創傷 / 炎症制御 / microRNA

Outline of Final Research Achievements

Diabetes is known to delay the healing of wounds and cause complications such as foot ulcers. We have previously shown that the retention of neutrophils in diabetic cutaneous wounds is aberrant in mice, inflammation is prolonged, and the pathogenesis may be due to dysfunction of diabetes-derived neutrophils. MicroRNAs (miRNAs) are critical regulator for normal inflammatory response. Accordingly, in order to clarify the molecular mechanism of regulation of inflammation in diabetes-derived neutrophils, we identified specific miRNAs in these cells using microarrays and analyzed the function of miRNAs in diabetes-derived neutrophils. In this study, we identified specific miRNA in neutrophil of type 2 diabetes mouse using microarray, and clarified a part of functions of inflammation-related miRNA and its target genes using cellular and molecular biological.

Free Research Field

法医学