2016 Fiscal Year Final Research Report
Passage culture of monocyte-macrophage lineage cells
| Project/Area Number |
15K20317
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plastic surgery
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| Research Institution | Kyorin University |
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Principal Investigator |
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| Research Collaborator |
KURITA Erina 杏林大学, 医学部, 専攻医
SHIOTA Noriko 杏林大学, 医学部, 実験助手
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 単球・マクロファージ系細胞 / 継代培養 / 創傷治癒 |
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| Outline of Final Research Achievements |
We established a passage culture of monocyte-macrophage lineage cells using a temperature-responsive cell culture dish. Macrophage colony-stimulating factor increased proliferation of the cells during the passage culture. The rate of monocyte-macrophage lineage cells (CD11b+/CD14+) increased by passages, and the cells were mostly purified by three passages. The cultured monocyte-macrophage lineage cells were positive for CD206, a marker of tissue-resident macrophages, and secreted various cytokines such as TNF-alpha and MCP-1.
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| Free Research Field |
創傷治癒
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