Functional analysis of adipose-derived stem cells and its application to cultured epidermal sheets

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Juntendo University
• Principal Investigator: Iida Hideo 順天堂大学, 医学部, 非常勤助教 (30751078)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脂肪組織由来幹細胞 / 表皮角化細胞 / 培養表皮シート / ケラチン / コラーゲン

Outline of Final Research Achievements

Adipose-derived stem cells were co-cultured with dermal fibroblasts on type IV collagen in Dulbecco’s modified Eagle’s medium containing all-trans retinoic acid (ATRA) and bone morphogenetic protein 4 (BMP4). Next, the cells were co-cultured with dermal fibroblasts in keratinocyte serum-free medium (KSFM) lacking ATRA and BMP4, followed by culturing in KSFM on type IV collagen in the absence of other cells. Approximately, 80% of these differentiated cells stained positive for keratin 10, although undifferentiated cells did not. It was thought that 45% of these cells differentiated into keratinocyte-like cells.
These differentiated cells were seeded, cultured, and lifted to the air-liquid interface, on fibroblasts-embedded type I collagen gel covered with type IV collagen. However, it was impossible to prepare a 3D culture cell sheet with sufficient strengths for clinical application. It was necessary to develop appropriate scaffolds.

Free Research Field

再生医学