Tau protein as a diagnostic biomarker for diffuse axonal injury

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20334
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Chiba University
• Principal Investigator: TOMITA Keisuke 千葉大学, 医学部附属病院, 医員 (80568668)
• Research Collaborator: NAKADA Takaaki 千葉大学, 医学部附属病院, 講師
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: びまん性軸索損傷 / タウ蛋白 / バイオマーカー

Outline of Final Research Achievements

DAI is a severe TBI,associated with poor outcome whose mortality exceeds 40%.However,diagnostics of DAI has not yet been established,especially in the early phase after injury.We hypothesized that serum level of tau protein,which was localized in neuronal axons, would be a useful biomarker for diagnosis and severity assessment of DAI.To elucidate relationship between severity of DAI and serum tau protein concentration,using an animal model. We evaluated association between serum concentration of tau and severity of DAI using a rat model of DAI.DAI was induced by dropping a weight in the head from a predetermined height(mild DAI group;1m,severe DAI group;2m).Eighteen rats were divided into 3 groups,sham-operated(n=4),mild DAI(n=7)and severe DAI(n=7).Serum levels of tau protein were measured by enzyme linked-immunosorbent assay.The serum levels of tau at 60 minutes after injury were statistically different among three groups(sham<mild<severe,sham vs.mild,p=0.024;mild vs.severe,p=0.018)

Free Research Field

救急集中治療医学