The expression of NETs and responsiveness of neutrophils in patients with ARDS

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20341
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Osaka University
• Principal Investigator: Hirose Tomoya 大阪大学, 医学系研究科, 特任研究員 (70597509)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: NETs / 好中球 / 感染 / ARDS / 低酸素下

Outline of Final Research Achievements

Neutrophil extracellular traps (NETs) is one of the immune systems to suppress dissemination of infection by the netted chromatin decorated with antibacterial molecules. We hypothesized that NETs production was differenced by patients and the concentration of oxygen. First, we exmained neutrophils in healthy volunteers and compared NETs production under atmospheric oxygen and hypoxia. Blood samples were obtained from healthy volunteers. A total of five volunteers cooperated in this study. NETs was produced by PMA stimulation under both conditions. NETs amount decreased under hypoxia in the four cases and the average reduction rate was 57.8 %; however, it was not statistically significant (p=0.13).These results indicated that NETs production might be reduced under low concentration of oxygen. Further analysis has to be made to clarify the relations between NETs production and oxygen by neutrophils in patients with sepsis and ARDS.

Free Research Field

救急・集中治療