2016 Fiscal Year Final Research Report
Mechanism analysis for evasion of periodontal pathogen from host innate immunity
| Project/Area Number |
15K20360
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 歯学 |
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| Outline of Final Research Achievements |
<Objective> In this study, we focused on the molecular basis underlying intracellular localization of Porphyromonas gingivalis (Pg), a pathogenic bacterium of periodontitis, in gingival epithelial cells. It has been reported that Rab4A, one of the Rab GTPases, has a key role in the fast recycling from early endosomes to plasma membrane. The objective of this study is to determine the involvement of Rab4A in intracellular localization of Pg. <Results> At 1-3 hour after infection, about one third of intracellular Pg was co-localized with Rab4A and RUFY1 in human immortalized gingival epithelial cells (HIGECs). Knockdown of Rab4A increased viable Pg in HIGECs, and caused accumulation of Pg in early endosomes even up to 5 h after infection. <Conclusion> Rab4A is suggested to be involved in the intracellular trafficking of Pg from early endosomes to distinct organelles in gingival epithelial cells.
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| Free Research Field |
口腔衛生学
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