Identification and virulence analysis of surface proteins in Aggregatibacter actinomycetemcomitans

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20365
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Kagoshima University
• Principal Investigator: Oogai Yuichi 鹿児島大学, 医歯学域歯学系, 助教 (40511259)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 歯周病原因菌 / 表層タンパク

Outline of Final Research Achievements

In this study, we tried virulence analysis of surface proteins on periodontal pathogen Aggregatibacter actinomycetemcomitans. To analysis the exposed proteins on outer membrane, trypsin shaving was performed. As the results, several surface proteins including previously known virulence factors Omp100 and EmaA were identified. However, many of the identified proteins were intracellular located proteins such as the ribosomal proteins, chaperons and enzymes. These results indicate some of the identified intracellular proteins are derivative from killed bacteria by trypsin treatment. Improved protocol is needed to systematic identification of surface proteins in this microbe.

Free Research Field

口腔細菌学