Induction of odontoblast from iPS cells for novel dental restorative method.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20412
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Tokyo Dental College
• Principal Investigator: Nakajima Kei 東京歯科大学, 歯学部, 助教 (20733463)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: iPS細胞 / 分化誘導 / 象牙芽細胞 / 象牙質

Outline of Final Research Achievements

The purpose of this study was to investigate odontoblast induction and differentiation from iPS cells, and dentin formation using induced cells in vivo. To induce for odontoblast, embryonic bodies (EB) were formed from iPS cells and cultured with retinoic acid and BMP-4. It was suggested that inducted cells were odontoblasts by PCR and immunohistochemistry. It was shown that differentiation into odontoblasts significantly decreased in SOX11 knockdown group. Transplantation of induced odontoblasts into mice was formed bone-like hard tissues, however dentin formation was not clear. These results indicate that SOX11 is considered as an important upstream control gene in odontoblast differentiation.

Free Research Field

医歯薬学