Development of cytokine, cell adhesion protein conjugate type DNA scaffold materials

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20420
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Conservative dentistry
• Research Institution: Fukuoka Dental College
• Principal Investigator: Yamamoto Nana 福岡歯科大学, 口腔歯学部, 助教 (60736677)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: DNA / DNAジェリー / サイトカイン / 細胞接着タンパク質

Outline of Final Research Achievements

We thought that utilize DNA as biomaterial. There is an osteoplasty promotion function, and DNA has the characteristic that it becomes Jerry of the porosity if mixed with water by adjusting quantity of addition because it is porous. DNA Jerry, and it was injectable. DNA specifically couples cationic protamine in the helical structure. Protamine which let cytokine and cell adhesion protein covalently link in a cross linker to DNA electrostatically, and DNA lets scaffold materials. It was difficult to establish the technique that a conjugaion. Examined a correlation of ingredient of the additive and new osteoplasty ability from in vitro, an in vivo examination, and DNA having the environment that promotes osteoplasty. The new osteoplasty ability the bone defect on the rat skull with DNA Jerry and recognized the new bone when observed it in microCT . The cytotoxicity was relatively mild when performed HE dyeing. It was suggested that use alone acted on predominance for new bone.

Free Research Field

歯周病学