Investigation of hypoxic response on oral mucosa keratinocytes focusing on HIF1-alpha expression for use in regenerative medicine

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20476
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Niigata University
• Principal Investigator: Kato Hiroko 新潟大学, 医歯学系, 助教 (70749994)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 口腔粘膜 / 上皮細胞 / 再生医療 / 低酸素 / HIF-1α

Outline of Final Research Achievements

Oral mucosa keratinocytes cultured in hypoxia showed higher proliferation than control, whereas cells treated with DMOG or HIF-1α siRNA in hypoxic culture showed no difference in low calcium medium. Then we analyzed the relationship between HIF-1α expression level and maintenance of undifferentiation state observing the undifferentiation marker; α6 integrin by western blot. Cells cultured in hypoxia or treated with DMOG showed higher expression of α6 integrin than control, however, cells treated with HIF-1α siRNA in hypoxic culture showed about the same level with control. Therefore, it is indicated that HIF-1α expression might regulate the maintenance of undifferentiated state of oral mucosa keratinocytes.

Free Research Field

歯科医用工学・再生歯学