Identification of hTERT supressor genes located on human choromosome 3

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20524
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Tottori University
• Principal Investigator: Nishio Sachiyo 鳥取大学, 医学部, プロジェクト研究員 (80726737)
• Research Collaborator: Kugoh Hiroyuki ; Ohira Takahito
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: テロメレース / 口腔がん / 染色体 / TERT

Outline of Final Research Achievements

Telomerase is crucial of cellular immortalization and cancer progression. Telomerase activity is primarily regulated by the expression of telomerase reverse transcriptase (TERT) which is a protein catalytic subunit of telomerase. In this study, we performed that introduction of a normal human chromosome 3 into OSCC cell line, HSC3, using microcell-mediated chromosome transfer (MMCT). As results, these hybrid clones also showed remarkable transcriptional suppression of TERT expression compared with parental HSC3 cells. To identify the gene responsible for the regulation of TERT transcription, we performed RNA-sequence analysis using parental HSC3 cells, telomerase negative HSC3 microcell hybrids with a chromosome 3 (HSC3#3) and its revertant clones (HSC3#3R) with reactivated telomerase. As a result, we identification of 11 candidate genes which as novel TERT suppressor genes.

Free Research Field

口腔腫瘍学