A development of gene delivery system that enable to making early diagnosis and inhibiting metastasis.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20540
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Kyushu University
• Principal Investigator: Kotaro Ishii 九州大学, 大学病院, 助教 (60596386)
• Research Collaborator: SUGIURA Tsuyoshi 鹿児島大学, 顎顔面疾患制御学分野, 教授 (40322292)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 口腔癌 / 転移機構

Outline of Final Research Achievements

1.　We established a lymph node metastatic subline from a highly metastatic AdCC cell line. DNA microarray hybridization and scanning were performed to compare RNA expression in lymph node metastatic cells with highly metastaticcells. The expression level of NNMT (Nicotinamide N-methyltransferase ) was up-regulated by 2000 times in lymph node metastatic cells. NNMT is known as a tumorigenesis agent. It was suggests that NNMT is involved in the metastasis in the AdCCs.
2.We performed miRNA microarray to identify differential miRNAs in serum pools of 6 OSCC patients and 6 health control. Then, we analyzed expression levels of miRNA in each serum sample of OSCC patients and control by real time PCR. MiR-183 showed significant over expression in patient group and was concerned with metastasis.

Free Research Field

口腔外科学