2016 Fiscal Year Final Research Report
Analysis of mechanotransduction of Scleraxis expression in the periodontal ligament
Project/Area Number |
15K20577
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Orthodontics/Pediatric dentistry
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Research Institution | Tohoku University |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2017-03-31
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Keywords | Scleraxis / 歯根膜細胞 / メカノトランスダクション |
Outline of Final Research Achievements |
In this study, we applied tensile force to human PDL fibroblasts to analyze relationship between Scleraxis (Scx), a transcription factor for tendon/ligament cell lineages, and TGF-β/Smad signaling.Scx mRNA expression was significantly up-regulated at 3 h. We also detected activation of p-Smad3. Pretreatment with SB-431542 or SIS3 showed not only inhibition of phosphorylation of Smad3 in a dose-dependent manner but also down-regulation of Scx mRNA. In conclusion, up-regulation of Scx mRNA in response to tensile force is regulated via TGF-β/Smad signaling.
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Free Research Field |
歯科矯正学
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