2016 Fiscal Year Final Research Report
Role of IGF-I in morphogenesis of bioengineered teeth
| Project/Area Number |
15K20579
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 再生歯胚 / IGF-I / エナメル結節 |
|---|
| Outline of Final Research Achievements |
We reported that the IGF-I treatment increased the size and cusp number of bioengineered teeth. However, the biological mechanisms of IGF-I on the bioengineered teeth morphology remain unclear. In this study, we examined the biological mechanisms underlying the effect of IGF-I on the bioengineered teeth morphology. Microarray analysis was performed using dental epithelial cells derived from tooth germs. The IGF-I treated dental epithelial cells expressed high level of various factors which are expressed during tooth development. Moreover, the proliferation and differentiation of the IGF-I treated dental epithelial cells were significantly upregulated. The number of the enamel knot increased in the IGF-I treated bioengineered teeth. These data suggest that IGF-I is involved in the biological mechanisms during tooth development.
|
| Free Research Field |
医歯薬学
|
