2016 Fiscal Year Final Research Report
Development of novel caries suppression method targeting polymer binding domain of plaque constituting bacteria
| Project/Area Number |
15K20591
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Orthodontics/Pediatric dentistry
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | Streptococcus mutans / グルカン結合タンパク / 齲蝕 / バイオインフォマティクス |
|---|
| Outline of Final Research Achievements |
Oral bacteria bind to other bacterial organisms and molecular proteins for biofilm formation. Streptococcus mutans, implicated as the primary causative agent of dental caries in humans, produces multiple glucan-binding proteins (Gbps), which are considered to promote formation of biofilm on tooth surfaces. In our previous study, we used bioinformatics to identify the glucan-binding domain sequence DPTKTIF, which is located in the middle of the gbpC gene encoding GbpC. In the present study, we constructed anti-DPTKTIF serum, and examined its effects on biofilm formation and binding to saliva proteins. Biofilm formation by S. mutans in the presence of anti-DPTKTIF serum was lower than that in its absence. In addition, anti-DPTKTIF serum was shown to bind to lysozyme. Our results suggest that an anti-DPTKTIF agent may be useful for inhibition of S. mutans adherence to the pellicle on tooth surfaces, resulting in inhibition of biofilm formation.
|
| Free Research Field |
医歯薬学
|
