Identification of novel genes involved in osteoblastogenesis of human periodontal ligament-derived mesenchymal stromal cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20617
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Health Sciences University of Hokkaido (2016-2017); Tokyo Medical and Dental University (2015)
• Principal Investigator: YAMADA AZUSA 北海道医療大学, 歯学部, 助教 (30708847)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 間葉系幹細胞 / 骨芽細胞分化

Outline of Final Research Achievements

To identify the mechanism of osteoinductive medium (OIM)-induced osteoblastic differentiation of human periodontal ligament-derived mesenchymal stromal cells (hPDL-MSCs), whole transcriptome analysis and RT-PCR were performed. As a result,
EGF like domain multiple 6 (EGFL6) was suggested to be involved in osteoblastic differentiation of hPDL-MSCs. Transfection of siEGFL6 significantly up-regulated the ALP activity of hPDL-MSCs in all samples. Knockdown of EGFL6 enhanced the expression of osteogenic genes, such as RUNX2, BSP, and OPN. Therefore, EGFL6 might be a novel regulator of osteogenesis in hPDL-MSCs.

Free Research Field

歯周組織再生学