2016 Fiscal Year Final Research Report
Development of an epigenome-editing technology for inducing cancer epimutations in mice
Project/Area Number |
15K20888
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory animal science
Tumor biology
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Research Institution | University of Tsukuba |
Principal Investigator |
ISEKI Hiroyoshi 筑波大学, 国際統合睡眠医科学研究機構, 助教 (50433652)
|
Project Period (FY) |
2015-04-01 – 2017-03-31
|
Keywords | エピゲノム編集 / CRISPR/Cas9 / lncRNA |
Outline of Final Research Achievements |
In this study, we attempted to develop an epigenome-editing technology by applying a CRISPR/Cas9 system and epigenome-modifying enzyme-scaffold proteins and lncRNAs. We first generated a functional small dCas9 mutant by deleting the N-terminal half of the dCas9 protein. Next, we developed an epigenome-editing system with epigenome-modifyling enzyme-scaffold proteins.We also identified a candidate epigenome-modifying enzyme-scaffold lncRNA.
|
Free Research Field |
発生工学、実験動物学
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