Functional roles of protein deubiquitinase regulated by ncRNAs

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20890
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science; Molecular biology
• Research Institution: National Center for Global Health and Medicine
• Principal Investigator: Naganuma Takao 国立研究開発法人国立国際医療研究センター, その他部局等, 上級研究員 (40466462)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 非コードRNA / 脱ユビキチン化酵素 / RNA結合 / 脱ユビキチン化

Outline of Final Research Achievements

We found that USP10, which is the one of deubiquitinase, interacts with some ncRNAs and mRNAs, and identified the RNA binding region of USP10. By analyzing the precise interaction between USP10 and HNRNPK, we also found that RNAs mediate the interaction between them. The knockdown of USP10 with siRNA showed that USP10 deubiquitinates HNRNPK within the cell. However, both the significance that USP10 interacts with RNAs and the functional roles that USP10 deubiquitinates HNRNPK within the cell remain to be elucidated.

Free Research Field

RNA生物学