Development of new procedure to make chimeric animals without using naive pluripotent stem cells

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20948
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Laboratory animal science
• Research Institution: The University of Tokyo
• Principal Investigator: Masaki Hideki 東京大学, 医科学研究所, 助教 (20571988)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ES細胞 / iPS細胞 / 再生医療 / 臓器再生 / キメラ

Outline of Final Research Achievements

Our group had shown that forced expression of anti-apoptotic factor enables developmental stage mismatched cells, such as primed state pluripotent stem cells or endodermal progenitor cells, to form chimera with pre-implantation mouse embryos. In this research, we tried to clarify this approach is also applicable for non-rodent cells, and/or other lineage progenitor cells except for endodermal progenitor cells. As a result, we observed that rabbit ESCs formed chimera with mouse and rabbit pre-implantation embryo by forced expression of anti-apoptotic gene, whereas control rabbit ESCs did not form any chimeras. We also observed heart region specific chimera formation with Nkx2.5 expressing cardiomyocyte progenitor cells by preventing apoptosis. Therefore, we conclude that promotion of chimera formation via inhibition of apoptosis can be applicable not only for rodent, and not only for endodermal progenitors.

Free Research Field

発生生物学、分子細胞生物学