2015 Fiscal Year Research-status Report
Mechanism of autoinflammation in chronic granulomatous disease: the molecular crosstalk between innate immune cells and gastrointestinal environments
| Project/Area Number |
15K20949
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| Research Institution | The University of Tokyo |
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Principal Investigator |
頼 貞儀 東京大学, 医科学研究所, 特任研究員 (30739925)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Autoinflammation / CGD |
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| Outline of Annual Research Achievements |
Chronic granulomatous disease (CGD) is basically a phagocyte disorder, but is also characterized by autoinflammation in gastrointestinal tract and it remains to be the problem. We hypothesize that innate lymphoid cells (ILCs) may play roles on CGD colitis. To verify this, first, the hematopoietic stem and progenitor cells (HSPCs), the origin of ILCs, was examined in a X-CGD murine model. Results showed HSPCs in CGD mice comparable to control, suggesting that progenitor cell differentiation remained unaffected. Second, the induced pluripotent stem (iPS) cells derived from a CGD patient were established. The results obtained from mouse model will be translated to human CGD-iPS cells, it is of important since we are aiming at establishment of treatment for CGD colitis via regulation of ILCs in patients.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
The research was slightly delayed for the reason that production of X-CGD mice did not works so well due to the aging of the carrier female. To deal with this, the mating strategy was switched to producing the new generation carrier females by using male X-CGD mice and wild-type female mice.
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| Strategy for Future Research Activity |
The research will be carried as previously planed once the new generation X-CGD mice become available. Even through the progress in FY2015 was slightly delayed due to the lack of X-CGD mice, the production of human CGD-iPS cells which was planed to be established in FY2016 was done in advance. Therefore, during the waiting period of X-CGD mice production, the downstream experiments using CGD-iPS cells will also proceed simultaneously.
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| Causes of Carryover |
The reason for incurring FY2015 research funding to the next year is that the research progress was slightly delayed due to the production of X-CGD mice did not work so well. Therefore, only a part of the funding in FY2015 was used.
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| Expenditure Plan for Carryover Budget |
The incurred amount of funding will be used to purchase the experimental animals and materials as initially planned.
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