Hybrid Raman-bioluminescence imaging for the study of proteome-metabolome interaction

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20950
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry; Physical chemistry
• Research Institution: The University of Tokyo
• Principal Investigator: Chiu Liang-da 東京大学, 大学院理学系研究科(理学部), 特任助教 (80648220)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: Metabolome analysis / Raman spectroscopy / Anti-Stokes fluorescence / Differentiation / メタボローム解析 / ラマン分光法 / アンチストークス蛍光 / 分化

Outline of Final Research Achievements

Within the 2-year funding period, I have developed a novel imaging method that can acquire the Raman and fluorescence images of living cells in one single scan. Conventional methods required separate scans for the Raman and fluorescence modes, which is not suitable for the study of living specimen that constantly move or change its chemical condition due to metabolism. The hybrid fluorescence-Raman imaging platform has been further applied to study the differentiation process of embryonic stem (ES) cells. The hybrid imaging platform was able to extract the subtle chemical change in the ES cells according to the expression pattern of Oct-4, a differentiation marker protein that is expressed only in undifferentiated stem cells.

Bioluminescent probes were planned for the original study, but they were out-performed by fluorescent probes due to the difference in emission intensity. The hybrid imaging platform is now a powerful platform to study the metabolism of live-cells.

Free Research Field

バイオイメージング