Photo-activatable PEG-Lipid surface for versatile and high-throughput patterning of multi-types of cells at single-cell resolution

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20963
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess; Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokyo
• Principal Investigator: YAMAHIRA Shinya 東京大学, 大学院工学系研究科(工学部), 特別研究員 (70750652)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: PEG脂質 / ケージド化合物 / 多種細胞パターニング / 血中循環がん細胞 / 光応答性化合物 / 界面科学

Outline of Final Research Achievements

The technology of patterning of multi-types of cells has expected as a powerful tool in next-generation cell research. In this study, we developed a photo-activatable PEG-lipid which can immobilize cells by light irradiation, as a molecule that enables multi-patterning of cells. On the surface modified with this molecule, versatile and high-throughput patterning of multi-types of cells were performed by repeating a process of light irradiation, seeding of arbitrary types of cells and washing. Additionally, by light-trapping only the target cells on this surface, it was possible to capture and isolate important cells such as circulating cancer cells from peripheral blood.

Free Research Field

材料工学