2016 Fiscal Year Final Research Report
Development of the method for evaluation of lipid absorption and gut immunology by using the mesenteric lymph cannulated rat model.
| Project/Area Number |
15K21037
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Eating habits
Food science
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 腸間膜リンパ管 / カニュレーション / 免疫機能 |
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| Outline of Final Research Achievements |
To elucidate the food-intake dependent relationship between lipid absorption and gut immunology, we developed the mesenteric lymph cannulated rat model. The all experiment was carried out under continuous isoflurane anesthesia. The lymph was collected from one hour before the administration of food sample (distilled water or cocoa butter) to four hours later of the administration. The lymph flow was increased in a short period after the administration of distilled water, however in the case of cocoa butter, it was increased 3-4hr later of the administration. It was not observed a big change in the ratio of T cell and B cell of the collected lymph. Interestingly, the IL-22 concentration of the collected lymph was 10 to 20 times higher than that of serum plasma. To elucidate the significance of IL-22 concentration in the lymph, further experiment will be needed.
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| Free Research Field |
リンパ動態学
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