High-throughput analysis of olfactory receptor repertoire by using the automated single-cell analysis and isolation system

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21068
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; Cell biology
• Research Institution: Osaka University
• Principal Investigator: Yoshimoto Nobuo 大阪大学, 産業科学研究所, 特任准教授 (80467612)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: １細胞解析 / 嗅覚細胞 / 嗅覚受容体 / 匂い分子 / 嗅覚受容体レパトア / バイオセンサー / 全自動１細胞解析単離装置

Outline of Final Research Achievements

A cell array consisting of 200,000 olfactory epithelial cells was formed on a microchamber array chip enabling cell alignment. After installing this cell array chip in the automated single-cell analysis and isolation system equipped with a perfusion system, the cells were subjected to time-lapse cytometry. Olfactory cells which transiently responded to stimulation of specific odor molecules were identified, and then the desired olfactory cells were isolated at single cell level. Each isolated single cell was subjected to genetic analysis to identify a combination of olfactory receptor groups that bind odor molecules specifically.

Free Research Field

生物工学