Expanding human iPS cell-derived alveolar type II cells and their application to disease modeling of hereditary pulmonary fibrosis

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology; Respiratory organ internal medicine
• Research Institution: Kyoto University
• Principal Investigator: Gotoh Shimpei 京都大学, 医学研究科, 准教授 (50747219)
• Research Collaborator: HOTTA Akitsu ; ASAKA Isao
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 再生医学 / iPS細胞 / 呼吸器 / 肺線維症 / II型肺胞上皮細胞

Outline of Final Research Achievements

Efficient generation and expansion of human iPS cell-derived alveolar type II cells was achieved. The rate of SFTPC+ cells per total epithelial cells reached around 50 % and iPS cell-derived SFTPC+ cells were long-term cultured for more than three months. We applied these methods to disease modeling of Hermansky-Pudlak Syndrome. Abnormal lamellar body structure was observed in human iPS cell-derived alveolar type II cells. Human iPS cell-derived alveolar type II cells would be beneficial for disease modeling of pulmonary fibrosis.

Free Research Field

呼吸器内科