Design and preparation of the fusogenic proteoliposomes by cell-free protein synthesis / liposome system

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K21119
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy; Biomedical engineering/Biomaterial science and engineering
• Research Institution: Kyoto University
• Principal Investigator: Ando Mitsuru 京都大学, 工学研究科, 特定研究員 (70737460)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 膜融合性膜タンパク質 / 無細胞タンパク質合成 / プロテオリポソーム / 膜融合

Outline of Final Research Achievements

We designed the fusogenic membrane protein-expressing plasmid DNAs to achieve the development of newly encapsulation methods and drug delivery systems based on membrane proteins reconstituted liposomes, proteoliposome. The cell-free fusogenic membrane protein synthesis was performed in the presence of liposomes. The fusogenic membrane proteins were successfully integrated into lipsosomal membrane, and we then evaluated the their fusion activities. Unfortunately, we failed to obtain the fusogenic proteoliposome in the present study. These findings indicated that it is necessary to add other molecular chaperons or regulate the post-translational modification to form the correct conformation of fusogenic membrane proteins in the present system.

Free Research Field

生物薬剤学