Dynamics of transgene for effective utilization of recombinant microorganisms by methane fermentation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21150
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental conscious materials and recycle; Applied microbiology
• Research Institution: Kobe University
• Principal Investigator: Sasaki Daisuke 神戸大学, 科学技術イノベーション研究科, 特命助教 (00650615)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 高温メタン発酵 / 組換え遺伝子 / 水平伝播 / 環境拡散 / 廃菌処理 / 廃棄物処理 / GFP遺伝子

Outline of Final Research Achievements

The possibility of horizontal gene transfer and environmentally spread of an exogenous gene into microbial communities was investigated by introducing yeast which constantly expresses recombinant gene (green fluorescent protein) in thermophilic methane fermentation reactor. As a result of operation of the fermenter for a long period and molecular biological analysis and microbiological analysis, when dead or living yeast holding the recombinant gene were introduced into the reactor, the gene was immediately degraded. Therefore, it was revealed for the first time that neither horizontal gene transfer nor spread of exogenous gene occurs.

Free Research Field

応用微生物学