Novel Therapeutic Strategy using Direct Reprogramming Methods for Stroke

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurosurgery; General medical chemistry
• Research Institution: Okayama University
• Principal Investigator: Yamashita Toru 岡山大学, 医歯薬学総合研究科, 講師 (60644408)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 脳梗塞 / 細胞移植 / iN細胞 / iPS細胞 / ダイレクトリプログラミング

Outline of Final Research Achievements

We investigated the therapeutic effect of iNSC transplantation, and also monitored engrafted iNSCs after transplantation of undifferentiated murine iNSCs in ischemic striatum/cortex of the mouse brain.
We found that iNSC transplantation successfully improved the survival rate of stroke model mice with significant functional recovery from the stroke. The fate of engrafted iNSCs was that the majority of iNSCs had differentiated into astroglial cells but not into neural cells in both the sham-operated brain and the poststroke brain without forming a tumor up to 8 months after tMCAO.

Free Research Field

脳神経外科