2017 Fiscal Year Final Research Report
Screening of essential ICB associate factors for spermatogenesis to understand male infertility
| Project/Area Number |
15K21217
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
Animal production science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Research Collaborator |
Kato Yuzuru
Iwamori Naoki
Matsumoto Masaki
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 生殖細胞間架橋 / 精子形成 |
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| Outline of Final Research Achievements |
Deletion of intercellular bridge (ICB), which connects all germ cells, caused male infertility. But the function of ICB still remains unknown. To understand the significance of ICB in spermatogenesis, its associating factors were identified and the interactions among each identified protein were comprehensively profiled. Specific antibodies to selected target proteins were developed and used to analyze their localization in testis. As a result, a novel Ectoplasmic Specialization (EPS) associating protein, KIAA1210, was identified. The knockout mouse of KIAA1210 was successfully produced and is under analyses. The results from analyses of KIAA1210 deficient mice will bring deep understandings of interactions between ICB and the other cell junction in spermatogenesis.
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| Free Research Field |
生殖生物学
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