2016 Fiscal Year Final Research Report
Development of novel strategy for suppressing chronic inflammation in adipose tissues utilizing adipose-derived stem cells.
| Project/Area Number |
15K21251
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Metabolomics
Drug development chemistry
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| Research Institution | Kindai University (2016)
University of the Ryukyus (2015) |
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Principal Investigator |
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| Research Collaborator |
TATSUMI Kohei 近畿大学, 医学部, 講師 (70555432)
KAJI Hiroshi 近畿大学, 医学部, 教授 (90346255)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 間葉系幹細胞 / 慢性炎症 / 2型糖尿病 |
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| Outline of Final Research Achievements |
Adipose-derived stem cells (ADSCs) were obtained from mouse subcutaneous adipose tissues digested by collagenase and centrifugation. Next, anti-inflammatory effects of ADSCs on the adipose tissues were examined in vitro culture system. In brief, the adipocytes were cultured in the addition of ADSC culture supernatant and the cells were analyzed the expressions of good and bad adipokines. We found out bad adipokines were decreased, on the other hand, good adipokines were not changed by the addition of ADSC supernatant.
Further we examined the beneficial effect of ADSCs on the Type 2 diabetic mice. The mice were divided to two groups to be subjected to ADSC sheet transplantation and sham operation groups, respectively. After the operation,on Day 10, we performed the insulin tolerance test and found out the insulin resistance were recovered by ADSC transplantation compared with the sham operation.
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| Free Research Field |
再生医学
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