2017 Fiscal Year Final Research Report
Analysis of GATA4 dimarization during hypertrophic responses
| Project/Area Number |
15K21279
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Biological pharmacy
Cardiovascular medicine
|
|---|
| Research Institution | University of Shizuoka |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | GATA4 / 心肥大反応 / 二量体形成 |
|---|
| Outline of Final Research Achievements |
A zinc finger protein, GATA4, associates with an intrinsic histone acetyltransferase (HAT), p300, and regulates myocardial transcriptional activities in response to hypertrophic stimuli. In this study, we demonstrated whether GATA4 formed a homo-dimer and its functional mechanism. Chemical cross-linking assay using nuclear extract from HEK293T cells showed that GATA4 homo-dimer was increased by co-expression of p300. IP-WB assay demonstrated that co-expression of GATA4 with p300 increased the formation of its homo-dimer as well as its acetylation. HAT-deficient mutant of p300 and acetyl-deficient mutant of GATA4 did not increase this homo-dimerization as well as GATA4-acetylation. These results suggest that acetylation of GATA4 is required for the formation of GATA4 dimerization. Thus, this dimerization may regulate hypertrophy-response gene activations and the development of heart failure.
|
| Free Research Field |
分子生物学
|
