Identification of neural pathway for suckling stimulus using kisspeptin cell specific retrograde tracing

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21286
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General anatomy (including histology/embryology); Obstetrics and gynecology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Yamada Shunji 京都府立医科大学, 医学(系)研究科(研究院), 講師 (40454079)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 逆行性トレーサー / 狂犬病ウイルス / Cre / キスペプチン

Outline of Final Research Achievements

Although expression of kisspeptin in the arcuate nucleus (ARH) is remarkably inhibited in lactating rats who are suppressed reproductive function, the neural pathway have not been fully explored. To investigate the neurtal pathway of suckling stimulus, in the present sutdy, I tried to establish a tracing method visualizing only projecting neurons to first-infected cell with pseudotype rabies virus using Cre-FLEX system.
Since I visualized the projecting neurons to neuropeptide Y (NPY)-expressing neurons in the ARH in the NPY-Cre mice, it is considered that the tracing methods from the present study can be applied to an analysis of neural pathway for various neuropeptide including kisspeptin.

Free Research Field

神経内分泌