Development and finding the mechanism of pancreatic beta cell differentiation from hiPS cells through Single cell analysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K21333
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Life / Health / Medical informatics; Biomedical engineering/Biomaterial science and engineering
• Research Institution: National Center for Global Health and Medicine (2016-2017); Saitama Medical University (2015)
• Principal Investigator: Sugahara Izumi 国立研究開発法人国立国際医療研究センター, その他部局等, 研究員 (10633000)
• Research Collaborator: Okazaki Yasushi 埼玉医科大学, 医学部, 客員教授 (80280733) ; Matsumoto Masahito 埼玉医科大学, 医学部, 客員講師 (90321819) ; Nakachi Yutaka 埼玉医科大学, 医学部, 助教 (10522097) ; Watanabe Ami 東京大学, 定量生命科学研究所, 特任研究員 (40611421) ; Nikaido Itoshi 国立研究開発法人理化学研究所, 生命機能科学研究センター, ユニットリーダー (00383290)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: beta cell / single cell analysis / hiPS

Outline of Final Research Achievements

The human induced pluripotent stem cells are potential resource for type 1 diabetes cell based therapies. Although,the beta cell differentiation had been deeply investigated, the molecular mechanism of differentiation of beta cell from hiPSCs and in which levels were achieved the beta cell derived hiPSCs has not been understood completely. In this study we provide, a single cell transcriptome of differentiated beta cells from hiPSCs, in which double labelled with Venus and mCherry proteins (hIveNry). The Venus protein could express under INS promoter and mCherry under NGN3 promoter, meaning that by fluorescent protein could monitor from NGN3+ red endocrine progenitor to the Venus positive beta cells. In this study we performed the cell profiling of the fluorescent positive cells viewing the status of the differentiation of the beta cells in which the maturation process was confirmed by glucose responsivity.

Free Research Field

生物学