Develoment of MRSA rapid detection method using phage derived ligand protein

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K21360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Laboratory medicine; Infectious disease medicine
• Research Institution: Kitasato University
• Principal Investigator: Matsui Hidehito 北里大学, 北里生命科学研究所, 研究員 (80503797)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: MRSA / ファージ / 迅速診断

Outline of Final Research Achievements

Bacteriophages infect with a narrow range of host cells by binding on the specific cell-surface receptor. This high host-specificity is largely relies on the specific interaction of the ligand protein and the receptor molecule. This project aimed to develop a simple and rapid Staphylococcus aureus diagnosis test employing the phage ligand protein in the lateral flow assay. The ligand protein was expressed by the gene cloning technique and purified by the affinity chromatography. The test was able to detect 32 clinical isolates of S. aureus tested without exception and showed no cross reactivity with other microorganisms including 31 bacterial species and one strain of yeast so far tested. We previously developed a MRSA detection tool using a monoclonal antibody raised against PBP2’ in the immunochromatogaphy. The combination of the present method and the PBP2’ detection tool enabled to distinguish MRSA and MSSA.

Free Research Field

感染症学