2016 Fiscal Year Final Research Report
Identification of a novel gene for congenital anomalies of the kidney and urinary tract - the role of CBWD1 in the kidney development-
| Project/Area Number |
15K21385
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 腎臓発生 / 先天性腎尿路異常 |
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| Outline of Final Research Achievements |
Congenital anomalies of the kidney and urinary tract (CAKUT) is the most frequent cause of pediatric chronic kidney diseases. Better understanding of the pathogenesis requires identification of novel candidate genes. So far we had focused on a gene, CBWD1, as a candidate of a new CAKUT-causing gene. The principal aim of this study was to determine whether CBWD1 plays important roles in the kidney development. Kidney develops through the reciprocal interaction between two kinds of precurssors, metanephric mesenchyme and ureteric bud. To reveal the localization of Cbwd1 in the developmental kidney, we created cross sections of the kidneys at several developmenatl stages and performed immunohistochemistry using anti-Cbwd1 antibody. Then we found that the expression of Cbwd1 was increasing in ureteric bud cells from E13.5. Next we generated knockout mice for Cbwd1 and at now we are investigating the phenotype of Cbwd1(-/-) mice.
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| Free Research Field |
腎臓発生の基礎と臨床
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