2016 Fiscal Year Final Research Report
Mechanisms for synaptic localization of secreted synaptic cleft proteins
| Project/Area Number |
15K21489
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurophysiology / General neuroscience
Developmental biology
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| Research Institution | Toho University (2016)
Kyoto Sangyo University (2015) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | シナプス間隙 / 分泌性タンパク質 / シナプス発生 / アセチルコリン受容体 / コリン作動性シナプス / ショウジョウバエ |
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| Outline of Final Research Achievements |
Hikaru genki (Hig), a Drosophila secretory protein, specifically localizes to cholinergic synaptic clefts in the CNS and regulates the distribution of acetylcholine receptor (AchR). Hig diffuses extracellularly and is specifically captured at the synaptic clefts of cholinergic synapses. To reveal the mechanisms of synaptic localization of Hig, we identified novel synaptic cleft protein Hasp that is required for trapping of Hig at the synapses. Hasp is also secreted, diffused in the brain, and trapped by cholinergic synapses. Then we searched for Hasp-interacting proteins by LC-MS/MS analysis. High-resolution microscopy reveals that Hasp and Hig divide synaptic clefts into distinct compartments. These data provide insight into how Hasp and Hig construct the synaptic cleft matrix and regulate the differentiation of cholinergic synapses.
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| Free Research Field |
神経科学
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