Structural analysis of influenza virus segmented genome in the virion

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21607
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry; Molecular biology
• Research Institution: Microbial Chemistry Research Foundation
• Principal Investigator: TAKIZAWA Naoki 公益財団法人微生物化学研究会, 微生物化学研究所, 研究員 (50448502)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: インフルエンザウイルス / 分節化ウイルス / RNAウイルス / RNA-RNA結合

Outline of Final Research Achievements

The genome of influenza A virus consists of eight single-stranded negative-sense RNA segments. To be infectious virion, all eight segments need to be incorporated into a virion. However, the mechanism of how all eight segments are selectively incorporated into a virion is not known. To elucidate the mechanism, we try to construct a new system using chemical RNA cross-link reagent and next generation sequencing to reveal global RNA-RNA interactions between viral genome segments in virion. As a result, we find RNA-RNA interactions between specific segments in virion. This result will contribute to reveal the RNA interaction network between viral genome RNAs and the mechanism of specific incorporations of all eight segments.

Free Research Field

ウイルス学