2017 Fiscal Year Final Research Report
Structural and functional analyses of autophagy essencial protein Atg9
| Project/Area Number |
15K21608
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
Cell biology
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| Research Institution | Microbial Chemistry Research Foundation |
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Principal Investigator |
MATOBA Kazuaki 公益財団法人微生物化学研究会, 微生物化学研究所・構造生物学研究部, 研究員 (60613792)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | オートファジー / 膜タンパク質 |
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| Outline of Final Research Achievements |
To determine a structure of autophagy essential membrane protein, overexpression and solubilization conditions were established and the purity was high enough to be detected as a single band using CBB staining. Protein-Fab complex crystallization was performed, but structural analysis has not been the success. In order to observe the single particle by cryo-electron microscope, Nanodisc-protein-Fab complex was reconstituted. As a result, Fab bound particle was observed by negative staining.
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| Free Research Field |
構造生物学
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