Development of visualization method of phosphorylation dynamics of beta-catenin for the study of in vivo Wnt signal regulation machinery

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21625
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology; General anatomy (including histology/embryology)
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Takai Akira 国立研究開発法人理化学研究所, 生命システム研究センター, 基礎科学特別研究員 (60637205)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: Wntシグナル / シグナル伝達 / プローブ開発 / 発生生物学

Outline of Final Research Achievements

In this research, we aimed to develop novel visualization probes of phosphorylation dynamics of β-catenin for real-time and quantitative analysis of Wnt signaling at the level of β-catenin protein. As a result, we succeeded in the development of several probes with higher responsiveness to Wnt signaling, compared with conventional Wnt probe. After further improvement of these probes in S/N and sensitivity, we will apply these probes to quantitative analysis of in vivo Wnt signaling and study its regulation machinery.

Free Research Field

発生生物学、細胞生物学