Development of super-resolution microscopic live imaging method of histone modifications for understanding cell differentiation and reprogramming

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21629
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology; Biophysics
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Okamoto Kazuko 国立研究開発法人理化学研究所, 生命システム研究センター, 特別研究員 (40710265)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ヒストン修飾

Outline of Final Research Achievements

In this project, I aimed to develop a methodology, which combines photoactivated localization microscopy (PALM) and Fluorescence Resonance Energy Transfer (FRET) for obtain information of histone modification and its loci in living nuclei simultaneously. It has strongly suggested that histone modification regulates cellular states. Especially in the field of cell differentiation and reprogramming, the importance of histone modifications comes to be a big issue to understand the process of cell-state transition. The methodology will be a powerful method to clarify the dynamics of histone modification in various biological phenomena.

Free Research Field

細胞生物学