Evaluation of iPS cells using chromosome comformation capture analysis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K21630
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology; General medical chemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yamada Daisuke 国立研究開発法人理化学研究所, 統合生命医科学研究センター, 研究員 (50525897)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: iPS細胞 / ポリコーム / 染色体高次構造

Outline of Final Research Achievements

In this study, we reveal that polycomb protein Ring1b showed bi-functional role in somatic cell reprogramming to pluripotency. Indeed, we performed iPS induction assay using Ring1B KO B cell. We observed that Ring1B KO acceralates iPS induction in early phase, while inhibits in late phase. Furthermore, Ring1B deletion after iPS generation showed increasing of differentiated cells and decleationg of cell growth. Finally, enhaced 4C Circular chromosome conformation capture) -seq targeted in polycomb target gene, Ink4a/Arf (Cdkn2a), indicated that insufficient reprogramming were exist in iPS cells. These results suggested that polycomb can be used as a marker for iPS evaluation.

Free Research Field

応用分子細胞生物学